Prolyl Hydroxyproline is a biologically active matrikine dipeptide with the sequence Pro-Hyp found in collagen hydrolysates. Numerous studies have confirmed that oral intake of small collagen-derived peptides, particularly Pro-Hyp and Hyp-Gly, stimulates the growth and proliferation of dermal fibroblasts, leaving firming, anti-aging, and hydrating effects.
Studies with Prolyl Hydroxyproline have shown that it enhances hyaluronic acid production by skin fibroblasts and reduces transepidermal water loss (TEWL). Another trial showed that it also reduces glycation of skin structural proteins, enhances elasticity, and improves skin barrier function and insulin resistance, making it an advantageous ingredient for anti-aging applications.
Prolyl Hydroxyproline showed promising results in wound healing as a fibroblast growth-initiating and migration factor that plays an essential role in skin regeneration and has potential as a treatment for chronic wounds.
Like other similar peptides, it has delivery issues in topical applications. Pro-Hyp itself is not suitable for direct use in skincare products, and its acetylated analog, Acetyl Prolyl Hydroxyproline, has been used in some Japanese skincare products.Prolyl Hydroxyproline is included as a core peptide in an advanced peptide-based complex, called X50 Hyalufiller, where it is encapsulated in a PLGA/PVA polymeric shell, and guided with a fibroblast targeting peptide. In this system, it is effectively delivered into fibroblasts, which enables its anti-aging potential.
